3GD6

Crystal structure of divergent enolase from Oceanobacillus iheyensis complexed with phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	I 4 2 2
Unit cell lengths	117.006, 117.006, 134.741
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.910 - 1.600
R-factor	0.237
Rwork	0.236
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fyy
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.600
Rmerge	0.081
Number of reflections	61491
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.8M Potassium phosphate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K