3GD5

Crystal structure of ornithine carbamoyltransferase from Gloeobacter violaceus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	99.563, 142.423, 149.273
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.940 - 2.100
R-factor	0.24
Rwork	0.239
R-free	0.27100
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	Auto-Rickshaw
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.100
Rmerge	0.064
Number of reflections	123395
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	20% PEG 3350, 0.2M Sodium tartrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K