3GD3
Crystal structure of a naturally folded murine apoptosis inducing factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.050, 80.290, 419.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.640 - 2.950 |
| R-factor | 0.25095 |
| Rwork | 0.249 |
| R-free | 0.29965 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gv4 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.770 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 408.250 | 2.873 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.174 | 0.715 |
| Number of reflections | 49548 | |
| <I/σ(I)> | 1.7 | |
| Completeness [%] | 81.1 | 69.45 |
| Redundancy | 4.97 | 5.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 7 | 298 | 15% PEG 4000, 0.35M KNO3, pH 7.0, microbatch under oil, temperature 298K |
