3GCH

CHEMISTRY OF CAGED ENZYMES. BINDING OF PHOTOREVERSIBLE CINNAMATES TO CHYMOTRYPSIN

Experimental procedure

Spacegroup name	P 42 21 2
Unit cell lengths	69.700, 69.700, 97.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.900
R-factor	0.203
RMSD bond length	0.018
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000 *
High resolution limit [Å]	1.900 *
Rmerge	0.050 *
Number of reflections	9635 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	chymotorypsin	15 (mg/ml)
2	1	drop	sodium cacodylate	10 (mM)
3	1	drop	cetyltrimethylammonium bromide	0.75 (%sat)
4	1	drop	ammonium sulfate	45 (%sat)
5	1	reservoir	ammonium sulfate	65 (%sat)