3GCF
Terminal oxygenase of carbazole 1,9a-dioxygenase from Nocardioides aromaticivorans IC177
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 280.002, 161.661, 194.665 |
| Unit cell angles | 90.00, 118.65, 90.00 |
Refinement procedure
| Resolution | 48.910 - 2.300 |
| R-factor | 0.17446 |
| Rwork | 0.174 |
| R-free | 0.17508 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.310 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.910 | 48.910 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 319545 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 12% w/v PEG 8000, 0.4M lithium sulfate, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
