3GC0
Structure of the CMGC CDK Kinase from Giardia lamblia in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-30 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.240, 73.180, 75.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.300 - 2.000 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.26500 |
| Structure solution method | MR |
| Starting model (for MR) | 1oit modified by ccp4 program chainsaw |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.608 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.050 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 | 0.650 |
| Number of reflections | 20333 | |
| <I/σ(I)> | 16.44 | 2.3 |
| Completeness [%] | 99.1 | 92.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 290 | 20% PEG 3350, 200MM NA MALONATE, 0.4UL + 0.4UL PROTEIN AT 27 MG/ML, PH 6.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |
