3GBY
Crystal structure of a protein with unknown function CT1051 from Chlorobium tepidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-06 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.045, 38.784, 60.932 |
| Unit cell angles | 90.00, 95.06, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.660 |
| R-factor | 0.1818 |
| Rwork | 0.179 |
| R-free | 0.22632 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.545 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.096 | 0.826 |
| Number of reflections | 27379 | |
| <I/σ(I)> | 43.8 | 1.9 |
| Completeness [%] | 98.4 | 59.4 |
| Redundancy | 6.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2M Lithium sulfate, 0.1M HEPES, 25% w/v PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
