3GBT
Crystal structure of gluconate kinase from Lactobacillus acidophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-02-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.638, 96.008, 72.940 |
| Unit cell angles | 90.00, 93.32, 90.00 |
Refinement procedure
| Resolution | 40.079 - 2.400 |
| R-factor | 0.2009 |
| Rwork | 0.199 |
| R-free | 0.23060 |
| Structure solution method | SAD |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.146 | 0.606 |
| Number of reflections | 33170 | |
| <I/σ(I)> | 11.2 | 1.7 |
| Completeness [%] | 97.1 | 88.3 |
| Redundancy | 5.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 35% v/v tert-Butanol, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
