3GBR
Anthranilate phosphoribosyl-transferase (TRPD) double mutant D83G F149S from S. solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-12-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.725 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.945, 65.993, 84.262 |
| Unit cell angles | 90.00, 98.81, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.19383 |
| Rwork | 0.192 |
| R-free | 0.25954 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zyk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.080 | 2.300 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 31695 | |
| <I/σ(I)> | 21.3 | 5.1 |
| Completeness [%] | 98.7 | 97.6 |
| Redundancy | 3.37 | 3.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 18% PEG (w/v) 1500, 10% glycerol (v/v), 50mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
