3GBJ
Crystal structure of the motor domain of kinesin KIF13B bound with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.229, 86.520, 94.948 |
| Unit cell angles | 90.00, 106.80, 90.00 |
Refinement procedure
| Resolution | 39.062 - 2.102 |
| R-factor | 0.222 |
| Rwork | 0.221 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i6i |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.065 | 0.021 | 0.947 |
| Number of reflections | 133690 | ||
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 99.9 | 98.7 | 100 |
| Redundancy | 3.7 | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 18% PEG 3350, Ammonium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
