3GAA
The crystal structure of the protein with unknown function from Thermoplasma acidophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 184.318, 184.318, 260.485 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 101.020 - 2.700 |
| R-factor | 0.19923 |
| Rwork | 0.196 |
| R-free | 0.25556 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.067 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.020 | 2.768 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.140 | 0.670 |
| Number of reflections | 44396 | |
| <I/σ(I)> | 17.5 | 1.17 |
| Completeness [%] | 99.6 | 97.53 |
| Redundancy | 9.7 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 25% PEG4000,0.1M Mes, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
