3G9Q

Crystal structure of the FhuD fold-family BSU3320, a periplasmic binding protein component of a Fep/Fec-like ferrichrome ABC transporter from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR577A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-04
Detector	MAR CCD 165 mm
Spacegroup name	P 21 21 21
Unit cell lengths	35.517, 69.898, 114.169
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.120 - 2.600
R-factor	0.222
Rwork	0.222
R-free	0.29100
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.077	0.214
Number of reflections	16199
<I/σ(I)>	25.87	4.9
Completeness [%]	96.6	88.9
Redundancy	6.3	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	291	Protein solution: 10 mM Tris-HCl pH 7.5, 100 mM Sodium chloride, 5 mM DTT. Reservoir solution: 20% PEG 3350, 200 mM NaNO3, VAPOR DIFFUSION, SITTING DROP, temperature 291K