3G9P
GR DNA binding domain:Sgk 16bp complex-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.115878 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.382, 38.470, 97.039 |
| Unit cell angles | 90.00, 123.44, 90.00 |
Refinement procedure
| Resolution | 34.354 - 1.652 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.20290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1glu |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.487 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.077 | 0.644 |
| Number of reflections | 42923 | |
| <I/σ(I)> | 19.5 | 1.7 |
| Completeness [%] | 88.2 | 34.6 |
| Redundancy | 8.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 50mM MES, pH 6.0, 20mM MgCl2, 15% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 2 | MES | ||
| 4 | 1 | 2 | MgCl2 | ||
| 5 | 1 | 2 | isopropanol |
