3G9M
GR DNA-binding domain:Sgk 16bp complex-44
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.058, 38.713, 96.475 |
| Unit cell angles | 90.00, 123.71, 90.00 |
Refinement procedure
| Resolution | 30.613 - 1.610 |
| R-factor | 0.1875 |
| Rwork | 0.187 |
| R-free | 0.19920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1glu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.328 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.059 | 0.567 |
| Number of reflections | 42461 | |
| <I/σ(I)> | 18.7 | 1.6 |
| Completeness [%] | 90.4 | 55.8 |
| Redundancy | 4.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 50mM Tris, pH 7.5, 200mM KCl, 50mM MgCl2, 10% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Tris | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 2 | Tris | ||
| 5 | 1 | 2 | KCl | ||
| 6 | 1 | 2 | MgCl2 | ||
| 7 | 1 | 2 | PEG4000 |
