3G9G
Crystal Structure of the N-terminal EFC/F-BAR domain of Syp1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-22 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 52.940, 97.950, 138.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.370 - 2.400 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.24200 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 14372 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 99.1 | 98.4 |
| Redundancy | 6.7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 294 | 0.2M NaCitrate pH 5.6, 0.2M Ammonium acetate, 20% PEG-3350, 3% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
