3G9D
Crystal structure glycohydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-11-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 163.866, 163.866, 46.274 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| R-factor | 0.20207 |
| Rwork | 0.199 |
| R-free | 0.26638 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cwc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.193 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.076 | 0.200 |
| Number of reflections | 24409 | |
| <I/σ(I)> | 14.3 | 4.8 |
| Completeness [%] | 94.8 | 91.9 |
| Redundancy | 7.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Hepes, pH7.5, 15% PEG 20000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
