3G9C
Crystal structure of the product Bacillus anthracis glmS ribozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.586, 229.827, 101.750 |
Unit cell angles | 90.00, 90.16, 90.00 |
Refinement procedure
Resolution | 49.690 - 2.900 |
R-factor | 0.249 |
Rwork | 0.246 |
R-free | 0.30700 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.328 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
Rmerge | 0.138 | 0.091 | 0.965 |
Number of reflections | 59605 | ||
<I/σ(I)> | 13.198 | ||
Completeness [%] | 95.6 | 99.2 | 68.4 |
Redundancy | 6.5 | 7.4 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | vapor diffusion, sitting drops | 6.8 | 298 | 11% PEG 8000, 9% DMSO, 0.02M SODIUM CACODYLATE, 0.02M MAGNESIUM CHLORIDE, 0.15M POTASSIUM CHLORIDE, pH 6.8, vapor diffusion, sitting drops, temperature 298K |