3G9A

Green fluorescent protein bound to minimizer nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	77.2
Detector technology	CCD
Collection date	2008-09-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98137
Spacegroup name	P 21 21 21
Unit cell lengths	50.780, 81.620, 94.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.000 - 1.614
R-factor	0.1623
Rwork	0.161
R-free	0.19350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	1.710
High resolution limit [Å]	1.610	1.610
Number of reflections	49989
<I/σ(I)>	16.91	2.84
Completeness [%]	97.5	86.3
Redundancy	3.85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	100mM Mes pH 6.5, 30% PEG 8000, 15% Glycerol, VAPOR DIFFUSION, temperature 293K