3G96
Crystal structure of the Bacillus anthracis glmS ribozyme bound to MaN6P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.401, 232.369, 104.657 |
| Unit cell angles | 90.00, 90.65, 90.00 |
Refinement procedure
| Resolution | 34.750 - 3.010 |
| R-factor | 0.258 |
| Rwork | 0.255 |
| R-free | 0.31100 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.733 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.140 | 0.096 | 0.779 |
| Number of reflections | 41443 | ||
| <I/σ(I)> | 8.471 | ||
| Completeness [%] | 92.9 | 97.2 | 90.6 |
| Redundancy | 3.9 | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapor diffusion, sitting drops | 6.8 | 298 | 11% PEG 8000, 9% DMSO, 0.02M SODIUM CACODYLATE, 0.02M MAGNESIUM CHLORIDE, 0.15M POTASSIUM CHLORIDE, pH 6.8, vapor diffusion, sitting drops, temperature 298K |
