3G8X
GR DNA binding domain:GilZ 16bp complex-65
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.115869 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.213, 37.914, 96.993 |
| Unit cell angles | 90.00, 123.22, 90.00 |
Refinement procedure
| Resolution | 35.363 - 2.050 |
| R-factor | 0.2138 |
| Rwork | 0.209 |
| R-free | 0.25740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1glu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.323 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.137 | 0.456 |
| Number of reflections | 21522 | |
| <I/σ(I)> | 15 | 2.2 |
| Completeness [%] | 94.5 | 71.1 |
| Redundancy | 6.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 50mM Na Cacodylate, pH 7.0, 2.25mM Spermine, 9mM MgCl2, 1.8 CoCl2, 5% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Cacodylate | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 2 | Cacodylate | ||
| 4 | 1 | 2 | MgCl2 | ||
| 5 | 1 | 2 | CoCl2 | ||
| 6 | 1 | 2 | Spermine | ||
| 7 | 1 | 2 | PEG 8000 |
