3G8W
Crystal structure of a probable acetyltransferase from Staphylococcus epidermidis ATCC 12228
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929, 0.97940 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 96.960, 110.409, 220.515 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.390 - 2.700 |
| R-factor | 0.21542 |
| Rwork | 0.213 |
| R-free | 0.25626 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.650 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.093 | 0.759 |
| Number of reflections | 32550 | |
| <I/σ(I)> | 37.8 | 2.7 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 297 | 0.1M CHES, 1.0M Sodium citrate, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
