3G8S
Crystal structure of the pre-cleaved Bacillus anthracis glmS ribozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.364, 228.480, 103.915 |
| Unit cell angles | 90.00, 90.58, 90.00 |
Refinement procedure
| Resolution | 43.770 - 3.100 |
| R-factor | 0.24942 |
| Rwork | 0.246 |
| R-free | 0.30597 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.486 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.000 | 45.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 6.670 | 3.100 |
| Rmerge | 0.219 | 0.063 | |
| Number of reflections | 39374 | ||
| <I/σ(I)> | 5.844 | ||
| Completeness [%] | 99.4 | 98 | 98.4 |
| Redundancy | 4 | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 298 | 11% PEG 8000, 9% DMSO, 0.02M SODIUM CACODYLATE, 0.02M MAGNESIUM CHLORIDE, 0.15M POTASSIUM CHLORIDE, pH 6.8, vapor diffusion, sitting drops, temperature 298K, VAPOR DIFFUSION, SITTING DROP |
