3G8M
Serine Hydroxymethyltransferase Y55F Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97623 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 112.590, 112.590, 68.929 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.300 |
| R-factor | 0.25625 |
| Rwork | 0.255 |
| R-free | 0.27535 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dfo |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.776 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.130 | 0.480 |
| Number of reflections | 6600 | |
| <I/σ(I)> | 9.7 | 2.5 |
| Completeness [%] | 94.0 | 97.3 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.1M Hepes (N-(2-hydroxylethyl)piperazine-N -2 ethansulfonic acid) pH 7.5, 1.4M sodium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
