3G8L
Crystal structure of murine natural killer cell receptor, Ly49L4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 78.045, 78.045, 216.738 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.2279 |
| Rwork | 0.223 |
| R-free | 0.28719 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.045 |
| RMSD bond angle | 3.756 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.067 | |
| Number of reflections | 25636 | |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 50.2 | |
| Redundancy | 9.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 1.8M ammonium sulfate, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
