3G8B
T. thermophilus 16S rRNA G527 methyltransferase in complex with AdoMet in space group I222
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-05 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 110.103, 113.976, 132.677 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.820 - 2.100 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.20100 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.782 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.063 | 0.528 |
| Number of reflections | 48613 | |
| <I/σ(I)> | 3.6 | |
| Completeness [%] | 97.9 | 99.2 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 277 | 30% (v/v) methyl-2,4-pentanediol (MPD), microbatch technique under oil, temperature 277K, pH 6.8 |
