3G89
T. thermophilus 16S rRNA G527 methyltransferase in complex with AdoMet and AMP in space group P61
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-03 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 61 |
| Unit cell lengths | 77.212, 77.212, 167.807 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.700 - 1.500 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.21000 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.124 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.123 | 0.381 |
| Number of reflections | 84726 | |
| <I/σ(I)> | 2.16 | |
| Completeness [%] | 93.8 | 99.4 |
| Redundancy | 6.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 277 | 10% (w/v) PEG8000, 100 mM imidazole (pH 8.0), microbatch technique under oil, temperature 277K |
