3G80
Nodamura virus protein b2, RNA-binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.190, 56.600, 98.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 500.000 - 2.500 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2B9Z.pdb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.165 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.097 | 0.428 |
| Number of reflections | 6576 | |
| <I/σ(I)> | 6.3 | 1.7 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 15% PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
