3G7X
Female-specific histamine-binding protein 2, D24R mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.225, 78.728, 77.651 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.550 |
| R-factor | 0.18123 |
| Rwork | 0.179 |
| R-free | 0.21430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qft |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.305 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.300 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.059 | 0.540 |
| Number of reflections | 65264 | |
| <I/σ(I)> | 7.7 | 1.5 |
| Completeness [%] | 97.2 | 96.9 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 298 | protein at 10 mg/ml containing 2 mM Histamine, Reservoir contained 2.6 M Ammonium Sulphate, 0.1 M Bicine, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
