3G7R
Crystal structure of SCO4454, a TetR-family transcriptional regulator from Streptomyces coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.103, 59.129, 66.895 |
| Unit cell angles | 90.00, 101.38, 90.00 |
Refinement procedure
| Resolution | 27.390 - 1.380 |
| R-factor | 0.15078 |
| Rwork | 0.150 |
| R-free | 0.17364 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.064 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.390 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.079 | 0.377 |
| Number of reflections | 99408 | |
| <I/σ(I)> | 41.361 | 4.1 |
| Completeness [%] | 99.4 | 95.4 |
| Redundancy | 7.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2 M NaI, 20% PEG 3350, cryoprotected in 20% Ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
