3G7L
Chromodomain of Chp1 in complex with Histone H3K9me3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-09 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 1.08090 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 41.560, 41.560, 87.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.337 - 2.200 |
| R-factor | 0.2001 |
| Rwork | 0.196 |
| R-free | 0.23680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Chain A of pdb code 1KNE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.798 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.300 | 2.400 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.047 | 0.314 |
| Number of reflections | 7255 | |
| <I/σ(I)> | 14.87 | 3.46 |
| Completeness [%] | 97.9 | 99.8 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 0.05 M Zinc Acetate, 20% w/v PEG 3350, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
