3G7I
Crystal structure of a Delta class GST (adGSTD4-4) from Anopheles dirus, with glutathione complexed in one subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 50.378, 50.378, 272.049 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.050 |
| R-factor | 0.20235 |
| Rwork | 0.200 |
| R-free | 0.24827 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jlw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.201 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 26234 | |
| <I/σ(I)> | 36.552 | 11.94 |
| Completeness [%] | 98.5 | 94.1 |
| Redundancy | 9.4 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | 0.1M Sodium Cacodylate pH 6.6, 30% (w/v) PEG 4000, 0.15M Sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
