3G74
Crystal structure of a functionally unknown protein from Eubacterium ventriosum ATCC 27560
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918, 0.97940 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.996, 107.961, 67.691 |
| Unit cell angles | 90.00, 92.28, 90.00 |
Refinement procedure
| Resolution | 33.500 - 2.430 |
| R-factor | 0.21137 |
| Rwork | 0.209 |
| R-free | 0.26347 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.568 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.500 | 2.490 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.106 | 0.698 |
| Number of reflections | 30092 | |
| <I/σ(I)> | 24.3 | 1.83 |
| Completeness [%] | 99.1 | 98.4 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 2.5M Ammonium sulfate, 0.1M Sodium acetate trihydrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
