3G6V
DNA synthesis across an abasic lesion by human DNA polymerase-iota
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-05 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 98.150, 98.150, 203.315 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.220 - 2.200 |
| R-factor | 0.21399 |
| Rwork | 0.211 |
| R-free | 0.25146 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fll |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.266 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.058 | 0.428 |
| Number of reflections | 29220 | |
| <I/σ(I)> | 37.6 | 37.6 |
| Completeness [%] | 97.0 | 100 |
| Redundancy | 10.1 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Crystals grew over a period of one day, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
