3G6U
GR DNA-binding domain:FKBP5 16bp complex-49
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Detector technology | CCD |
| Collection date | 2007-04-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.115872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.243, 39.088, 95.610 |
| Unit cell angles | 90.00, 122.67, 90.00 |
Refinement procedure
| Resolution | 34.375 - 1.900 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1glu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.507 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 | 0.466 |
| Number of reflections | 27530 | |
| <I/σ(I)> | 20.7 | 1.6 |
| Completeness [%] | 72.6 | 72.6 |
| Redundancy | 8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 50mM HEPES, pH 7.5, 2.5mM Spermine, 80mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Hepes | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 2 | Hepes | ||
| 6 | 1 | 2 | MgCl2 | ||
| 7 | 1 | 2 | Spermine |
