3G6T
GR gamma DNA-binding domain:FKBP5 16bp complex-34
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-31 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.115799 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.693, 39.716, 95.228 |
| Unit cell angles | 90.00, 122.79, 90.00 |
Refinement procedure
| Resolution | 31.086 - 1.900 |
| R-factor | 0.1814 |
| Rwork | 0.179 |
| R-free | 0.21420 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.481 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.292 |
| Number of reflections | 26432 | |
| <I/σ(I)> | 32.6 | 2.1 |
| Completeness [%] | 90.3 | 44.3 |
| Redundancy | 3.2 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 50mM HEPES, pH 7.0, 100mM KCl, 10mM MgCl2, 5% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Hepes | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 2 | Hepes | ||
| 5 | 1 | 2 | KCl | ||
| 6 | 1 | 2 | MgCl2 | ||
| 7 | 1 | 2 | PEG 400 |
