3G6K
Crystal Structure of Candida glabrata FMN Adenylyltransferase in complex with FAD and Inorganic Pyrophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97874 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 206.583, 81.481, 136.603 |
| Unit cell angles | 90.00, 129.79, 90.00 |
Refinement procedure
| Resolution | 32.190 - 1.350 |
| R-factor | 0.155 |
| Rwork | 0.153 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fwk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.521 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 380943 | |
| <I/σ(I)> | 35.939 | 2.37 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7.4 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 22% (w/v) PEG MME 2000, 0.2 M Magnesium sulfate, 0.1 M Sodium acetate, pH 4.8, Temperature 293K, VAPOR DIFFUSION, HANGING DROP |
