3G6D
Crystal structure of the complex between CNTO607 Fab and IL-13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 115 |
| Detector technology | CCD |
| Collection date | 2006-12-22 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.540, 68.030, 182.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.200 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.26342 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 3G6A 1ijz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.361 |
| Data reduction software | d*TREK (9.6L) |
| Data scaling software | d*TREK (9.6L) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.161 | 0.220 |
| Number of reflections | 13028 | |
| <I/σ(I)> | 6.1 | 4.6 |
| Completeness [%] | 91.5 | 88.2 |
| Redundancy | 6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 293 | 0.1 M Sodium acetate pH 4.5, 4.9 M Sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
