3G6B
Crystal structure of a Soluble Chemoreceptor from Thermotoga maritima Asn217Ile mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.353, 25.698, 119.920 |
| Unit cell angles | 90.00, 94.13, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| Rwork | 0.259 |
| R-free | 0.30500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 1.258 |
| RMSD bond angle | 0.010 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 8972 |
| <I/σ(I)> | 22.5 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
