3G5W

Crystal structure of Blue Copper Oxidase from Nitrosomonas europaea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.91933
Spacegroup name	P 1
Unit cell lengths	76.140, 76.254, 105.145
Unit cell angles	81.48, 73.38, 61.84

Refinement procedure

Resolution	48.280 - 1.900
R-factor	0.168
Rwork	0.166
R-free	0.19700
RMSD bond length	0.008
RMSD bond angle	1.146
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.500	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.083	0.342
Number of reflections	153165
<I/σ(I)>	16.9	4.8
Completeness [%]		98.2
Redundancy	24.3	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	277	40% MPD, 4% Glycerol, pH 7.5, vapor diffusion, temperature 277K
2