3G5V
Antibodies Specifically Targeting a Locally Misfolded Region of Tumor Associated EGFR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.925, 83.161, 72.214 |
| Unit cell angles | 90.00, 92.43, 90.00 |
Refinement procedure
| Resolution | 41.581 - 2.001 |
| R-factor | 0.229 |
| Rwork | 0.226 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.115 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.282 |
| Number of reflections | 27692 | |
| Completeness [%] | 96.6 | 79.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 0.2M ammonium acetate, 16-18% PEG5000 monomethylether, EVAPORATION, temperature 293K |
