3G5R

Crystal structure of Thermus thermophilus TrmFO in complex with tetrahydrofolate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-23
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	46.809, 97.023, 53.794
Unit cell angles	90.00, 101.29, 90.00

Refinement procedure

Resolution	35.841 - 1.600
R-factor	0.1689
Rwork	0.168
R-free	0.18820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g5s
RMSD bond length	0.004
RMSD bond angle	0.943
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	35.850
High resolution limit [Å]	1.600
Number of reflections	60724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	20% PEG 8000, 0.2M calcium acetate, 0.1M Mes-NaOH (pH 6.5), 0.3mM tetrahydrofolic acid, VAPOR DIFFUSION, temperature 298K