3G5Q

Crystal structure of Thermus thermophilus TrmFO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	68.355, 75.370, 156.089
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.477 - 2.102
R-factor	0.152
Rwork	0.149
R-free	0.20020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g5s
RMSD bond length	0.007
RMSD bond angle	1.096
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.477
High resolution limit [Å]	2.100
Number of reflections	23205

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	298	13% PEG 3350, 0.2M ammonium citrate tribasic, 0.1M Bicine-NaOH (pH 8.0), VAPOR DIFFUSION, temperature 298K