3G5J
Crystal structure of N-terminal domain of putative ATP/GTP binding protein from Clostridium difficile 630
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-29 |
| Detector | ADSC QUANTUM Q315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.489, 60.192, 79.107 |
| Unit cell angles | 90.00, 108.62, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.760 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.19800 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.553 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.078 | 0.415 |
| Number of reflections | 40514 | |
| <I/σ(I)> | 18.5 | 2.2 |
| Completeness [%] | 94.5 | 59.3 |
| Redundancy | 2.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 292 | 0.2 M Lithium citrate, 20% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
