3G5G

Crystal Structure of the Wild-Type Restriction-Modification Controller Protein C.Esp1396I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9330
Spacegroup name	P 65
Unit cell lengths	128.717, 128.717, 137.508
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.984 - 2.800
R-factor	0.239
Rwork	0.237
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.579
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.984	3.000
High resolution limit [Å]	2.800	2.800
Rmerge	0.135	0.372
Number of reflections	31686	5939
<I/σ(I)>	16.8	7.7
Completeness [%]	99.5	93.7
Redundancy	11.17

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	precipitation	8	277	150 mM NaCl, 40 mM Tris-HCl, 5 % w/v glycerol, 2.5 mM CaCl2, pH 8.0, precipitation, temperature 277K