3G4P
OXA-24 beta-lactamase at pH 7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93300 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 102.310, 102.310, 84.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.950 - 1.970 |
| R-factor | 0.19917 |
| Rwork | 0.197 |
| R-free | 0.24408 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jc7 |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.479 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.590 | 2.080 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.086 | 0.509 |
| Number of reflections | 32064 | |
| <I/σ(I)> | 7.2 | 1.5 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 11.7 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M SODIUM ACETATE, 28% PEG 2000 MME, 0.1M HEPES (PH 7.5), VAPOR DIFFUSION, SITTING DROP, temperature 293K |
