3G40
Crystal structure of the cytoplasmic domain of a prokaryotic cation chloride cotransporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.964 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.045, 68.045, 137.025 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.907 - 1.900 |
| R-factor | 0.202 |
| Rwork | 0.198 |
| R-free | 0.23300 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.058 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 28259 | |
| <I/σ(I)> | 20.2 | 3.6 |
| Completeness [%] | 95.6 | 74.6 |
| Redundancy | 6.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1M MES, 1.1M MgSO4, pH6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
