3G3S

Crystal structure of GCN5-related N-acetyltransferase-like protein (ZP_00874857) (ZP_00874857.1) from Streptococcus suis 89/1591 at 1.80 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-15
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162, 0.97821, 0.97879
Spacegroup name	P 21 21 21
Unit cell lengths	71.395, 83.880, 93.763
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.285 - 1.800
R-factor	0.205
Rwork	0.203
R-free	0.23900
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.576
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.285	29.285	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.108	0.045	0.753
Total number of observations		2125	13918
Number of reflections	52108
<I/σ(I)>	4.81	11.6	1
Completeness [%]	98.8	96.9	98
Redundancy	3.7	3.2	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	NANODROP, 0.20M MgCl2, 10.0% PEG 3000, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K