3G3P
The structure of the M53A Mutant of the Caulobacter crescentus CLPS in complex with a peptide containing an amino-terminal norleucine residue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-09-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.665, 54.049, 44.615 |
| Unit cell angles | 90.00, 110.44, 90.00 |
Refinement procedure
| Resolution | 20.902 - 1.478 |
| R-factor | 0.1697 |
| Rwork | 0.169 |
| R-free | 0.19110 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.825 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.4_129) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.480 | 3.190 | 1.480 |
| Rmerge | 0.038 | 0.038 | 0.068 |
| Number of reflections | 22484 | ||
| <I/σ(I)> | 61.523 | ||
| Completeness [%] | 89.3 | 96.7 | 48 |
| Redundancy | 6.8 | 6.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 300 | 0.1 M bis-tris, 0.2 M MgCl2, 13% PEG 3350, pH 5.5, VAPOR DIFFUSION, temperature 300K |
