3G3O
Crystal structure of the cytoplasmic tunnel domain in yeast Vtc2p
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9780, 0.97790 0.9742 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.200, 74.590, 80.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.295 - 2.100 |
| R-factor | 0.2004 |
| Rwork | 0.199 |
| R-free | 0.23570 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.480 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.400 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 25562 | |
| <I/σ(I)> | 16.1 | 3.8 |
| Completeness [%] | 99.3 | 95.6 |
| Redundancy | 7.6 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 22 % PEG 4000, 0.2 M Li2SO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
