3G36
Crystal structure of the human DPY-30-like C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.401, 51.388, 51.388 |
| Unit cell angles | 90.00, 107.58, 90.00 |
Refinement procedure
| Resolution | 43.160 - 1.200 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.22700 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.353 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.300 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.048 | 0.345 |
| Number of reflections | 51431 | |
| <I/σ(I)> | 16.7 | 4.1 |
| Completeness [%] | 98.1 | 79.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1M Bis-Tris, 28% PEG-MME 2000, 3% 1,6 hexanediol (additive), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
